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The gastrointestinal tract is a diverse and complex ecosystem shaped by continual interactions between host cells, nutrients, and the gut microbiota. Under homeostatic conditions, the human gut microbiota limits the growth of pathogenic bacteria and restricts the influence of resident pathobionts. However, when the gut microbiota network is disrupted, pathobionts and pathogens can colonize and cause disease. This session focuses on factors which influence the colonization of Clostridioides difficile and other gut pathobionts.